Mako Analysis™
High-Resolution Immunogenomics From NGS Data
Mako delivers phased MHC haplotypes, 4-field HLA typing with extended coverage across 45 genes, and comprehensive immune region variant calls from NGS data.
Performance Where Standard Methods Fall Short
Instead of treating the MHC as a narrow HLA-calling problem, Mako is built for the most complex regions of the genome, like MHC, and reconstructs haplotypes before calling variants and HLA alleles.
Phased MHC Haplotypes
Mako reconstructs haplotypes across the MHC, enabling fine-mapping, association studies, and clearer interpretation
High-resolution
HLA Typing
Mako delivers 4-field resolution HLA typing with extended coverage across 45 genes in the MHC.
SNPs & SVs
across the MHC
Mako goes beyond classical HLA genes to recover dense variant calls across the entire MHC
Simple Workflow, Superior Results
Mako combines capabilities that are typically split across multiple tools in one short-read workflow: phased haplotype reconstruction, high-resolution HLA typing, and comprehensive immune-region variant calling.
Performance That Matters
In benchmark comparisons across the MHC, Mako outperformed standard short-read analysis with stronger variant calling performance in complex immune regions and accuracy of HLA allele calling.
Mako variant calling performance compared to DeepVariant and ground truth from custom long-read sequencing in the MHC delta block
Mako HLA calling concordance compared to T1K and HLA-HD in 11 medically relevant HLA genes (HLA-A, B, C, DPA1, DPB1, DQA1, DQB1, DRB1, DRB3, DRB4, DRB5)
Supporting Critical Research Applications
For research teams working in immunology, translational genomics, or population-scale studies, the broader completeness from Mako can mean clearer signals and more usable downstream results.
Translational Medicine Teams
For biomarker discovery, immunotherapy response studies, vaccine response genetics, and immune-related adverse event research.
Biobanks & Population Genomics Cohorts
For re-analysis of existing datasets, MHC fine-mapping, cross-ancestry studies, and immune genetic architecture work.
CROs and Genomics Service Providers
For organizations that want to add immunogenomics capability without building and maintaining a specialized MHC analysis pipeline.
Mako Frequently Asked Questions
Let’s Resolve Your Most Complex Genomic Regions
Get in touch to try our platform on your own microarray or NGS data.